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J. Biochem, 1986, Vol. 99, No. 5 1545-1548
© 1986 Japanese Biochemical Society

other

Marked Effect of Cations on the Separation of Spinach Thylakoid Chlorophyll-Proteins on Polyacrylainide Gel Electrophoresis at 0°C in the Presence of Alkanolammonium Dodecyl Sulfates1

Kanenobu KUBO* and Toshio TAKAGI**

*Faculty of Pharmacy, Kinki University Higashiosaka, Osaka 577
**Institute for Protein Research, Osaka University Suita, Osaka 565

The separation of spinach thylakoid chlorophyll-proteins on polyacrylamide gel electrophoresis at 0°C in the presence of dodecyl sulfate is markedly influenced by the kind of surfactant cation in the media used for solubilization and electrophoresis. The mode of separation can thus be modulated through the cation selection. Three kinds of alkanolammonium dodecylsulfates were tested and their abilities as to the dissociation of the molecular assemblies of chlorophyll-proteins were found to decrease in the following order: tris(hydroxymethyl)methylammonium, triethanolammonium and triisopropanolammonium. Comparison of the electrophoretic patterns obtained with different kinds of cation may help clarify the hierarchy of the molecular assemblies of chlorophyll-proteins.

1Supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan, to T.T. (Grant No. 60470157).


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