Skip Navigation



Journal of Biochemistry Advance Access published online on April 4, 2008
Journal of Biochemistry, doi:10.1093/jb/mvn042
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Han, M.
Right arrow Articles by Ning, Q.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Han, M.
Right arrow Articles by Ning, Q.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Japanese Biochemical Society

The nucleocapsid protein of SARS-CoV induces transcription of hfgl2 prothrombinase gene dependent on C/EBP alpha

Meifang Han*,{dagger}, Weiming Yan*,{dagger}, Yuancheng Huang*,{dagger}, Huaning Yao*,{dagger}, Zhanhui Wang§, Dong Xi*,{dagger}, Weina Li*,{dagger}, Yaoyong Zhou*,{dagger}, Jinlin Hou§, Xiaoping Luo{ddagger},{dagger} and Qin Ning*,{dagger},2

*Department of Infectious Disease, {ddagger}Department of Pediatrics, {dagger}Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China, §Department of Infectious Disease, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.

2Correspondence to Dr Qin Ning, Department of Infectious Disease, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China. Tel & Fax: 86-27 83662391, Email: qning{at}tjh.tjmu.edu.cn

Received February 4, 2008; Accepted March 11, 2008


  Abstract

Fibrin deposition was universal in the lungs of SARS patients and fgl2 prothrombinase gene, a novel procoagulant, was demonstrated to express highly in a clinically relevant SARS model. To investigate whether and which structural protein of SARS-CoV induced transcription of hfgl2 prothrombinase gene, three eukaryotic expression plasmids expressing nucleocapsid protein (N), membrane protein (M) and spike protein 2 (S2) of SARS-CoV were cotransfected with hfgl2 promoter luciferase-reporter plasmids and β-galactosidase plasmid in CHO cells, respectively. M, N and S2 protein of SARS-CoV were detected by Western blotting and immunohistochemistry analysis. Further assays demonstrated that expression of hfgl2 gene was related with N protein, but not with M or S2 protein in THP-1 cells and Vero cells. N protein significantly induced functional procoagulant activity in comparison with control group. Luciferase assay showed that N protein of SARS-CoV could activate the transcription of hfgl2 promoter compared with the pcDNA3.1 empty vector. Site Directed mutagenesis and EMSA assay further demonstrated that transcription factor C/EBP alpha band with its cognate cis-element in hfgl2 promoter. The results showed that N protein of SARS-CoV induced hfgl2 gene transcription dependent on the transcription factor C/EBP alpha, which maybe contribute to the development of thrombosis in SARS.

Key Words: gene regulation, hfgl2, nucleocapsid protein, prothrombinase, SARS-CoV


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.