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Journal of Biochemistry Advance Access originally published online on June 24, 2009
Journal of Biochemistry 2009 146(3):439-447; doi:10.1093/jb/mvp091
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© The Authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

Storage of Gangliosides GM2 and Fucosyl GM1 in the Kidney of MCC Strain of Mastomys (Praomys coucha)

Kazuhiro Takimoto1,*, Nariko Kawamura2 and Takeshi Kasama3

1Division of Experimental Animal Research; 2Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640; and 3Instrumental Analysis Research Center for Life Science, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan

*To whom correspondence should be addressed. Tel: +81-3-5285-1111 ext. 2198, Fax: +81-3-5285-1267, E-mail: takimoto{at}nih.go.jp

Received March 26, 2009; Accepted June 11, 2009


  Abstract

Previously, we histochemically examined the kidney of the MCC strain of mastomys (Praomys coucha) and found the storage of gangliosides. In the present studies, the lipid-bound sialic acid content of gangliosides in the MCC kidney was about 9- to 14-fold higher than that of the control (MWC strain). In the MCC kidney, sialic acids of male gangliosides were composed of N-acetylneuraminic acid at 91.5%; sialic acids of female gangliosides, however, were composed almost entirely of N-glycolylneuraminic acid. TLC of gangliosides showed that the MCC kidney contained four abundant gangliosides (two gangliosides each in males and females). These gangliosides isolated by HPLC were identified to be GM2(NeuAc) and fucosyl GM1(NeuAc) in the male MCC kidney and GM2(NeuGc) and fucosyl GM1(NeuGc) in the female MCC kidney by secondary ion mass spectrometry, TLC/immunostaining and TLC after enzyme treatments. Although the MCC kidney contained control levels of the activities of β-N-acetylhexosaminidase, {alpha}-L-fucosidase, N-acetylgalactosaminyltransferase and fucosyltransferase, the activity of β-galactosidase in the MCC kidney was increased to 400–500% of that in the MWC kidney. Therefore, we discussed the possibility that in the MCC kidney, GM2 was abundantly produced by the effect of increased β-galactosidase activity.

Key Words: fucosyl GM1, ganglioside, GM2, kidney, mastomys

Abbreviations: Cer, ceramide; CDH, lactosylceramide; fucosyl GM1(NeuAc), IV2Fuc{alpha},II3NeuAc{alpha}-Gg4Cer; fucosyl GM1(NeuGc), IV2Fuc{alpha},II3NeuGc{alpha}-Gg4Cer; Hex, hexose; NeuAc, N-acetylneuraminic acid; NeuGc, N-glycolylneuraminic acid; GD1a, IV3NeuAc{alpha},II3NeuAc{alpha}-Gg4Cer; GD1b, II3NeuAc{alpha}2-Gg4Cer; GD2, II3NeuAc{alpha}2-Gg3Cer; GD3, II3NeuAc{alpha}2-LacCer; GM1(NeuAc), II3NeuAc{alpha}-Gg4Cer; GM1(NeuGc), II3NeuGc{alpha}-Gg4Cer; GM2(NeuAc), II3NeuAc{alpha}-Gg3Cer; GM2(NeuGc), II3NeuGc{alpha}-Gg3Cer; GM3, II3NeuAc{alpha}-LacCer; GM3(NeuGc), II3NeuGc{alpha}-LacCer; 4-MU, 4-methylumbelliferyl; PBS, phosphate-buffered saline; SI-MS, secondary ion mass spectrometry


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