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Journal of Biochemistry Advance Access published online on September 17, 2009
Journal of Biochemistry, doi:10.1093/jb/mvp147
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© The authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

Rapid Communication

Antisense transcription occurs at the promoter of a mouse imprinted gene, Commd1, on the repressed paternal allele.

Keiichiro Joh1,*,{dagger}, Hitomi Yatsuki1,{dagger}, Ken Higashimoto1, Tsunehiro Mukai2 and Hidenobu Soejima1,*

1Division of Molecular Genetics and Epigenetics, Department of Biomolecular Sciences, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501
2Saga University, 1 Honjo-machi, Saga 840-8502, Japan

*Corresponding authors: Keiichiro Joh, Tel: +81 952-34-2262; Fax: +81 952-34-2067 E-mail: joh{at}cc.saga-u.ac.jp. Hidenobu Soejima, Tel: +81 952-34-2260; Fax: +81 952-34-2067 E-mail: soejimah{at}cc.saga-u.ac.jp

Received July 16, 2009; Accepted September 1, 2009


  Abstract

The Commd1 gene is imprinted in the adult mouse brain and is predominantly expressed from the maternal allele. A paternally expressing imprinted gene, U2af1-rs1, resides in the first intron of Commd1 in an antisense orientation. We found that RNA polymerase II phosphorylated at serine 2 of the carboxyl-terminal domain repeats, a marker of transcription elongation, is enriched on the paternal allele than on the maternal allele of the Commd1 promoter. The Commd1 promoter harbors no allelic differences in DNA methylation and histone modifications. These results strongly suggested that imprinting of Commd1 is generated by interference with paternal Commd1 transcription by the oppositely directed U2af1-rs1 transcription.

{dagger}K. J. & H. Y. contributed equally to this work.


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