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Journal of Biochemistry Advance Access published online on October 20, 2009
Journal of Biochemistry, doi:10.1093/jb/mvp161
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© The authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

Dppa2 Knockdown-induced Differentiation and Repressed Proliferation of Mouse Embryonic Stem Cells

Juan Du+, Tianji Chen+, Xin Zou, Bo Xiong and Guangxiu Lu*

Institute of Reproduction and Stem Cell Engineering, Central South University, 110 Xiangya Road, Changsha, Hunan, China, 410078

* Corresponding author,FAX: +86-731-84497661, E-mail: lugxdirector{at}yahoo.com.cn

Received August 6, 2009; Accepted October 5, 2009


  Abstract

Developmental pluripotency-associated 2 (Dppa2) gene is one of the genes recently identified to be expressed specifically in pluripotent cells. To investigate the role of Dppa2 in mouse embryonic stem (ES) cells, we examined its expression during differentiation and performed knockdown of Dppa2 in mouse ES cells. Our results showed that the expression of Dppa2 decreased markedly in differentiated cells. Dppa2 knockdown induced the differentiation of mouse ES cells, as indicated by reduced alkaline phosphatase (AKP) activity, slightly downregulated expression of the putative pluripotency marker genes Oct4 and Nanog, and increased expression of early differentiation marker genes, such as Fst and Psx1. Moreover, reduced expression of Dppa2 also repressed cell proliferation activity as shown by the 5-bromo-2-deoxyuridine (BrdU) incorporation assay. Hence, Dppa2 might play a role in maintenance of the undifferentiated state and proliferation of ES cells.

Key Words: mouse ES cells, Dppa2, knockdown, undifferentiated state, proliferation

+ Both authors contributed equally to this work.


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