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Journal of Biochemistry Advance Access published online on November 6, 2009
Journal of Biochemistry, doi:10.1093/jb/mvp181
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© The authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

Importance of Tyr310 residue in the third repeat of microtubule binding domain for filament formation of tau protein

Chisato Nishiuraa, Kengo Takeuchia, Katsuhiko Minouraa,*, Miho Sumidab, Taizo Taniguchib, Koji Tomooa,* and Toshimasa Ishidaa

aOsaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan
bBehavioral and Medical Sciences Research Consortium, 2-5-7 Tamachi, Akashi, Hyogo 673-0025, Japan

*Corresponding author. Fax: +81-726-90-1068. E-mail: minoura{at}gly.oups.ac.jp

Received July 24, 2009; Accepted October 22, 2009


  Abstract

The inhibition of tau fibrillation is a potential therapeutic target for Alzheimer's and other neurodegenerative diseases. As a series of studies on inhibiting the transition of soluble monomeric tau into mature fibril, the effect of Tyr310 residue in the third repeat (R3) of the microtubule-binding domain (MBD) on the assembly of MBD was investigated using Tyr-substituted MBD mutants by fluorescence, circular dichroism spectroscopy, and electron microscopy. Consequently, the importance of the Tyr residue located at position 310, not at other positions, was clearly shown. The conformational comparison of the Tyr310Ala-substituted R3 repeat peptide with the unsubstituted one showed that the Tyr residue contributes to the rigid extended structure of the N terminal V306QIVYK311 sequence, and its replacement by Ala leads to the deformation of the extended structure, consequently losing its aggregation ability. The present results indicate that a compound that interacts specifically with the Tyr residue or an antibody recognizing the region containing the Tyr residue becomes a candidate for inhibiting tau fibrillation.

Key Words: Tau, Microtubule-binding domain, Tyrosine residue, Filament formation, Mutational analysis


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