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J. Biochem, 1986, Vol. 100, No. 4 1009-1014
© 1986 Japanese Biochemical Society

research-article

Characterization of Histamine Release in Digitonin-Permeabilized Rabbit Platelets

Isao ADACHI1, Chihiro SUMI, Kazuya FURUHASHI, Hirokazu ADACHI, Masaharu UENO and Isamu HORIKOSHI

Department of Hospital Pharmacy, Toyama Medical and Pharmaceutical University Toyama, Toyama 930-01

1To whom all correspondence should be addressed.

To manipulate the intracellular milieu of rabbit platelets, permeabilization was performed using digitonin. Permeabilized platelets showed dose-dependent release of histamine, which was stored in granules of rabbit platelets, in response to extracellular calcium ion. As PMA stimulated the release reaction in digitonin-permeabilized platelets, the protein kinase C system, which regulates metabolic processes and cell reactions in intact platelets, was revealed to be working. Cupric phenanthroline also released histamine from permeabilized rabbit platelets dose-dependently, and dithiothreitol inhibited the release strongly. Since cupric phenanthroline is a mild oxidant which catalyzes the formation of disulfide bridges, as in the case of Ca2+-ATPase of sarcoplasmic reticulum, the results suggested that protein crosslinking is implicated in the regulation of the release reaction in permeabilized rabbit platelets.


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