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J. Biochem, 1988, Vol. 103, No. 5 744-746
© 1988 Japanese Biochemical Society

other

Presence of Phosphatidylcholine Hydroperoxide in Human Plasma

Teruo Miyazawa, Faculty of Agriculture*,1, Keiichi Yasuda*, Kenshiro Fujimoto* and Takashi Kaneda**

* Department of Food Chemistry, Tohoku University Sendai, Miyagi 980
**Kohriyama Women's College Kohriyama, Fukushima 963

1To whom correspondence should be addressed.

A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was newly developed and used for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in human plasma. The method involves separation of phosphatidylcholine derivatives from plasma lipids by normal phase HPLC and subsequent detection of hydroperoxide-dependent chemiluminescence (CL) of PCOOH. CL was produced through luminol oxidation during the reaction of the hydroperoxide and cyto-chrome c-heme. The high specificity for the hydroperoxide allows the sensitive assaying of a large PCOOH range over a concentration range of 50–2,000 pmol of hydroperoxide-O2. Using this method, the occurrence of PCOOH in normal human plasma was strongly suggested and was confirmed quantitatively.


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