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J. Biochem, 1988, Vol. 103, No. 5 840-842
© 1988 Japanese Biochemical Society

research-article

Inhibitory Effect of Taurolipids on Clostridium perfringens Sialidase1

Keiko Nohara-Uchida and Kunimitsu Kaya

Basic Medical Sciences Division, The National Institute for Environmental Studies Tsukuba, Ibaraki 305

Taurolipids A and B, which are detergent-type compounds isolated from protozoan Tetrahymena cells, were demonstrated to inhibit strongly the activity of Clostridium perfringens sialidase. On addition of 280 pmol of taurolipid B to 20 mU of the enzyme, the sialidase activity was decreased to 7% of the original activity at pH 5.1 as the optimum pH. The inhibition was non-competitive. Effective inhibition was observed at the acidic region from the isoelectric point of the sialidase, and at a low ionic strength. Both the long chain acyl and sulfonic acid groups of taurolipids were required for the inhibition of the sialidase activity. A mechanism is postulated for the inhibition.

1This work was supported in part by a Grant-in-Aid for Scientific Research (No. 62780205) from the Ministry of Education, Science and Culture of Japan.


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