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J. Biochem, 1988, Vol. 104, No. 4 489-491
© 1988 Japanese Biochemical Society

other

Identification of Calcium Binding Proteins in Two-Dimensional Gel Electrophoretic Pattern of Drosophila Thorax and Their Distribution in Two Types of Muscles1

Yoshiharu Tanaka*, Kei Maruyama**, Takashi Mikawa*** and Yoshiki Hotta*

*Department of Physics, Faculty of Science Bunkyo-ku Tokyo 113
**Department of Pharmacology, Faculty of Medicine. The University of Tokyo Bunkyo-ku Tokyo 113
***Laboratory of Neurochemistry, National Institute for Physiological Sciecnce Okazaki, Aichi 444

Ten Drosophila thorax proteins (six myosin light chains and four proteins called a, b, c, d) were found to have high affinities with Ca2+ This was proved after subjecting the total Drosophila thorax proteins to two-dimensional (2D) transblot, followed by 45Ca2+ autoradiography. Three proteins (a, c, d) showed Ca2+ dependent electrophoretic mobility changes. To know their tissue-specific localization, fibrillar and tubular type muscle fibers were individually dissected from freeze-dried flies and separately subjected to 2D gel electrophoresis. Fibrillar type muscle had protein b and a small amount of protein a. Tubular type muscle had proteins c, d and a very large amount of protein a. Protein d was characterized to be calmodulin.

1 This study was supported by Grants-in-Aid to Y.H. from the Ministry of Education, Science and Culture of Japan.


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