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J. Biochem, 1988, Vol. 104, No. 4 495-497
© 1988 Japanese Biochemical Society


other

Liquid Chromatography/Mass Spectrometry of Fatty Acids as Their Anilides

Takashi Kusaka*, Mitsunori Ikeda*, Hiroyuki Nakano** and Yoko Numajiri***

*Biochemistry Department Kurashiki, Okayama 701-01;
**Biochemistry Center, Kawasaki Medical School Kurashiki, Okayama 701-01;
***Naka Works Hitachi, Ltd., Katsuta, Ibaraki 312

The mass spectra of a series of saturated (C16:0-C30:0) and unsaturated (C16:1 C18:1 C18:2 C18:3) fatty acids have been recorded as their anilides using liquid chromatography/mass spectrometry with the atmospheric-pressure-ionization interface system. The spectra show an intense peak for (molecule+H)plus ion in each case. The liquid chromatographic/mass spectrometric separation was performed on a reverse phase column using a solvent system of methanol alone or methanol + 2-propanol. This method seemed promising for application to both qualitative and quantitative micro-analysis of fatty acids including very long chain fatty acids.


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