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J. Biochem, 1988, Vol. 104, No. 4 509-511
© 1988 Japanese Biochemical Society

research-article

Cross-Linking of Native Myosin Forms Oligomers of Myosin Heavy Chain Dimers1

Di Hua Hu, Sumiko Kimura and Koscak Maruyama2

Department of Biology, Faculty of Science, Chiba University Chiba, Chiba 260

2To whom correspondence should be addressed

Chemical cross-linking of native myosin in 0.6 M NaC1 with p-phenylene bis maleimide or glutaraldehyde resulted in rapid formation of myosin heavy chain dimers and their oligomers. Monomers and odd-number oligomers disappeared after the prolonged treatment with these reagents. When denatured myosin was cross-linking, myosin heavy chain monomers and odd-number oligomers remained after the prolonged treatment, although dimers and their even-number oligomers were abundant. For high molecular weight markers, myosin heavy chain oligomers formed from denatured myosin with glutaraldehyde or p-phenylene bis maleimide are recommended.

1This work was supported by grants from the Ministry of Education, Science and Culture, the Ministry of Health and Welfare of Japan and the Muscular Dystrophy Association.


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