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J. Biochem, 1988, Vol. 104, No. 4 512-514
© 1988 Japanese Biochemical Society

research-article

Bovine High-Molecular-Weight Kininogen: A Revision of the Amino Acid Sequence of the Light Chain Portion1

Tatsuya Sueyoshi, Toshiyuki Miyata, Hisao Kato2 and Sadaaki Iwanaga3

Department of Biology, Faculty of Science Kyushu University 33, Higashi-ku, Fukuoka, Fukuoka 812

3To whom correspondence should be addressed

The amino acid sequence of the COOH-terminal light chain portion of bovine high-molecular-weight kininogen (HMWK) was reinvestigated, since some discrepancy of the amino acid sequence, which we reported previously, was found in the eDNA analysis for bovine HMWK (Kitamura, N. et al. (1983) Nature 305,545–548). The results showed that the following positions in the light chain should be revised: Gln-23 to Glu, the sequence Asp-Asp-Asp-Trp-Ser at positions 62–66 to Ser-Asp-Asp-Asp-Trp, Asp-102 to Asn, and Gln-109 to Glu.

1This study was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan. Portions of this paper (Tables IS-IIIS and Figs. 1S-6S) are presented in miniprint at the end of the paper.

2Present address: National Cardiovascular Center, Research Institute, Suita, Osaka 565.


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