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J. Biochem, 1988, Vol. 104, No. 4 521-525
© 1988 Japanese Biochemical Society

research-article

Studies on Nuclear Acid Deoxyribonucleases and Proofreading Exonuclease Isolated from Rat Liver Nuclei

Hiroto Akiyoshi

Department of Health Sciences, Faculty of Integrated Arts and Sciences, The University of Tokushima Tokushima, Tokushima 770

Three kinds of deoxyribonucleases (peaks A, B, and C) were separated from purified rat liver nuclei on DEAE-cellulose column chromatography, and their characteristics were partially studied. Peaks A and B had endonuclease activities under acidic conditions with low substrate specificity and did not require divalent metal ions. Peak C had an exonuclease activity under alkaline conditions with substrate specificity for denatured DNA or single stranded homopolymer and required divalent cations. Peak C degraded 3'-terminally mismatched substrate much faster than 3'-terminally matched substrate.


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