Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Akashi, A.
Right arrow Articles by Imamoto, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Akashi, A.
Right arrow Articles by Imamoto, F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1988, Vol. 104, No. 4 526-530
© 1988 Japanese Biochemical Society

research-article

Molecular Cloning and Expression of a Gene for a Factor Which Stabilizes Formation of Inhibitor-Mitochondrial ATPase Complex from Saccharomyces cerevisiae

Akira Akashi*, Yukuo Yoshida**, Hideki Nakagoshi***, Kazuyuki Kuroki***, Tadao Hashimoto**, Kunio Tagawa** and Fumio Imamoto***,1

*Department of Biochemistry Toagosei Chemical Industry Co., Ltd., Minato-ku, Nagoya, Aichi 455
**Department of Molecular Physiology, Osaka University Medical School Kita-ku, Osaka, Osaka 530
***Laboratory of Molecular Genetics, Riken (The Institute of Physical and Chemical Research) Tsukuba Life Science Center, Tsukuba, Ibaraki 305

1To whom correspondence should be addressed

Stabilizing factor, a 9 kDa protein, stabilizes and facilitates formation of the complex between mitochondrial ATP synthase and its intrinsic inhibitor protein. A clone containing the gene encoding the 9 kDa protein was selected from a yeast genomic library to determine the structure of its precursor protein. As deduced from the nucleotide sequence, the precursor of the yeast 9 kDa stabilizing factor contains 86 amino acid residues and has a molecular weight of 10,062. From the predicted sequence we infer that the stabilizing factor precursor contains a presequence of 23 amino acid residues at its amino terminus. We also used S1 mapping to determine the initiation site of transcription under glucose-repressed or derepressed conditions. These experiments suggest that transcription of this gene starts at three different sites and that only one of them is not affected by the presence of glucose.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
X. Wu, L. Zhu, J. Guo, D.-Y. Zhang, and K. Lin
Prediction of yeast protein-protein interaction network: insights from the Gene Ontology and annotations.
Nucleic Acids Res., January 1, 2006; 34(7): 2137 - 2150.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. J. Kominsky, M. P. Brownson, D. L. Updike, and P. E. Thorsness
Genetic and Biochemical Basis for Viability of Yeast Lacking Mitochondrial Genomes
Genetics, December 1, 2002; 162(4): 1595 - 1604.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Dienhart, K. Pfeiffer, H. Schagger, and R. A. Stuart
Formation of the Yeast F1F0-ATP Synthase Dimeric Complex Does Not Require the ATPase Inhibitor Protein, Inh1
J. Biol. Chem., October 11, 2002; 277(42): 39289 - 39295.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Vaillier, G. Arselin, P.-V. Graves, N. Camougrand, and J. Velours
Isolation of Supernumerary Yeast ATP Synthase Subunits e and i. CHARACTERIZATION OF SUBUNIT i AND DISRUPTION OF ITS STRUCTURAL GENE ATP18
J. Biol. Chem., January 1, 1999; 274(1): 543 - 548.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.