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J. Biochem, 1988, Vol. 104, No. 4 560-564
© 1988 Japanese Biochemical Society

research-article

Nonspecific Lipid Transfer Protein (Sterol Carrier Protein-2) Is Located in Rat Liver Peroxisomes1

Makoto Tsuneoka*, Akitsugu Yamamoto*, Yukio Fujiki** and Yutaka Tashiro*,2

*Department of Physiology, Kansai Medical University, Moriguchi Osaka 570
**Meiji Institute of Health Science Odawara, Kanagawa 250

2To whom correspondence should be addressed

Intracellular localization of nonspecific lipid transfer protein (nsLTP) in rat hepatocytes was investigated by immunoblot analysis of the subcellular fractions and immunoelectron microscopy, using affinity-purified antibody against nsLTP. Immunoblot analysis showed that the protein exists in the peroxisomal and cytosolic fractions. Further study indicated that nsLTP exists in the soluble subfraction of the peroxisomes. Immunoelectron microscopic observation revealed that nsLTP is highly concentrated in the matrices of the peroxisomes. From these results, we concluded that nsLTP mainly exists in the matrix of the peroxisomes. The role of nsLTP is discussed.

1Supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan and by Grants from Toray Science Foundation and the Japan Private School Promotion Foundation.


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