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J. Biochem, 1988, Vol. 104, No. 4 591-594
© 1988 Japanese Biochemical Society

research-article

Immunoaffinity Isolation of a Sialyl-Lea Oligosaccharide from Human Milk1

Hiroshi Kitagawa*, Hiroshi Nakada**, Yoshito Numata*, Akira Kurosaka**, Shigeyuki Fukui*, Ikuo Funakoshi*, Toshisuke Kawasaki*, Kazuo Shimada***, Fuyuhiko Inagaki*** and Ikuo Yamashina*,2

*Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University Sakyo-ku, Kyoto, Kyoto 606
**Division of Biological Engineering, Kyoto Sangyo University Kita-ku, Kyoto, Kyoto 603
***Department of Molecular Physiology, The Tokyo Metropolitan Institute of Medical Science Bunkyo-ku, Tokyo 113

2To whom correspondence should be addressed

A cancer-associated antigen, sialyl-Lea oligosaccharide, was isolated from human milk using a monoclonal antibody recognizing carbohydrate moieties of mucin-type glycoproteins. The structure was identified as

based on 500-MHz 1H-NMR spectroscopy. This oligosaccharide comprises 0.07% of sialyloligosaccharides in human milk. The NMR spectra of two fellow oligosaccharides, Lea oligosaccharide (or lacto-N-fucopentaose II) and LS-tetrasaccharide a are also given.

1This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan and by The Fugaku Trust for Medicinal Research.


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