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J. Biochem, 1988, Vol. 104, No. 4 610-615
© 1988 Japanese Biochemical Society

research-article

Detection of Nucleic Acid-Derived Radicals Formed by Alloxan

Toshihiro Ishii, Hideo Iwashahi, Ryojin Sugata and Ryo Kido1

Department of Biochemistry, Wakayama Medical College Wakayama, Wakayama 640

1To whom correspondence should be addressed

Pyrimidine base-derived radical spin adducts were detected in reaction mixtues containing pyrimidine bases, glutathione, and alloxan by the ESR spin trapping technique with a spin trap, {alpha}-phenyl-N-tert-butyl nitrone (PBN). Pyrimidine nucleoside- and nucleotide-, and ribose- and deoxyribose-derived radical spin adducts of PBN were also observed. However, purine base- and nucleoside-derived radical spin adducts of PBN were not detected. A cytosine-derived radical spin adduct of PBN was not generated under anaerobic conditions. Catalase and mannitol inhibited the formation of the cytosine-derived radical spin adduct of PBN but superoxide dismutase (SOD) did not. EDTA stimulated it and desferrioxamine suppressed it nearly completely. From these results it is presumed that the hydroxyl radical is involved in the formation of the cytosine-derived radical spin adduct of PBN generated by alloxan.


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