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J. Biochem, 1990, Vol. 107, No. 6 799-801
© 1990 Japanese Biochemical Society

other

The Blasticidin S Resistance Gene (bsr) Selectable in a Single Copy State in the Bacillus subtilis Chromosome

Mitsuhiro Itaya*,1, Isamu Yamaguchi**, Kaori Kobayashi***, Toyoshige Endo*** and Teruo Tanaka*

*Mitsubishi Kasie Institute of Life Sciences Machida, Tokyo 194
**The Institute of Physical and Chemical Research Wako, Saitama 351–01; and
***Kyoritsu College of Pharmacy Minato-ku, Tokyo 105

1 To whom correspondence should be addressed.

The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.


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