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J. Biochem, 1990, Vol. 107, No. 6 899-903
© 1990 Japanese Biochemical Society

research-article

Studies on Glycosphingolipids in Larvae of the Green-Bottle Fly, Lucilia caesar: Two Neutral Glycosphingolipids having Large Straight Oligosaccharide Chains with Eight and Nine Sugars1

Mutsumi Sugita*, Fuyuhiko Inagaki**, Hiromi Naito* and Taro Hori***

*Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University Otsu, Shiga 520
**Department of Molecular Physiology, The Tokyo Metropolitan Institute of Medical Science Bunkyo-ku, Tokyo 113
***Shiga Junior College of Culture and Technology Yokaichi, Shiga 527

Two neutral glycosphingolipids having large straight oligosaecharide chains with eight and nine sugars, provisionally named COS and CNS, were isolated and purified from larvae of the green-bottle fly, Lucilia caesar, as the only two remaining unidentified significant neutral glycolipids in this organism. From the results of sugar analysis, permethylation, negative-ion fast atom bombardment mass spectroscopy (FAB-MS), and 1H-NMR studies, the structures of the two glycolipids are proposed to be: COS, GalNAcßl-3GlcNAc/91-3Gal ßl-3GalNAcal-4GalNAcßl-4GlcNAc;91-3Manßl-4Glcßl-Cer; and CNS, Galßl-3GalNAcß l-3GlcNAc ß l-3Gal §l-3GalNAc {alpha} l-4GalNAc ß l-4GlcNAc ß l-3Man ß l-4Glc ß1-Cer. The fatty acid and long-chain base compositions of the above glycolipids were very similar, and were dominated by arachidic acid, and tetradeca- and hexadeca-4-sphingenines. The great similarity between the compositions of their ceramide moieties suggests that COS may be a precursor in the glycosylation reaction yielding.

1 This study was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan.


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