J. Biochem, 1990, Vol. 108, No. 1 37-41
© 1990 Japanese Biochemical Society
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Regulation of Activity of an ATP-Dependent Protease, Clp, by the Amount of a Subunit, ClpA, in the Growth of Escherichia coli Cells1
*Faculty of Agriculture, Tokyo University of Agriculture and Technology Fuchu, Tokyo 183
**Department of Chemistry, National Institute of Health Shinagawa-ku, Tokyo 141
The activity of an ATP-dependent protease, Clp, was examined in Escherichia coli SG1110 (lon–) in various growth phases. The ATP-dependent proteolytic activity (Clp activity) in a crude extract of the cells changed with the growth phase. Cells in the early exponential growth phase showed the lowest activity, but then the activity increased dramatically with cell growth. The highest Clp activity was found in the cells in the late exponential and early stationary phases, however, the activity returned to the original level on prolonged culturing. These changes in Clp activity were closely correlated to the amount of one of the components of Clp, Clp A, which was quantitated immunochemically with antibodies against the Clp A protein. However, the amount of the other component of Clp, Clp P, did not change with the growth phase. These results suggest that the activity of Clp in the cells is regulated by the amount of Clp A in various growth phases. We next examined the effect of the cellular ATP level on Clp activity, because ATP is a cofactor for Clp protease in vitro. The addition of dinitrophenol (DNP) and sodium azide reduced the intracellular concentration of ATP, but had no effect on the Clp activity or the level of the Clp A protein when these drugs were added to the culture at the stationary phase. On the other hand, these drugs elevated both the Clp activity and the Clp A amount in exponentially growing cells, whose cellular ATP level was also reduced. In this case, cell growth was inhibited by the drugs, suggesting that the reduction in the cellular ATP level is not directly correlated with elevation of the Clp A amount and the Clp activity, but indirectly through interruption of cell growth. These results suggest that Clp activity is regulated by the amount of the Clp A protein in vivo in E. coli cells, and that the Clp A amount varies with the cell growth phase.
1This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan, and The Agricultural Chemical Research Fundation.
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