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J. Biochem, 1990, Vol. 108, No. 1 86-91
© 1990 Japanese Biochemical Society

research-article

Maintenance of a Neutral Cytoplasmic pH Is Not Obligatory for Growth of Escherichia coli and Streptococcus faecalis at an Alkaline pH1

Shigeru Mugikura, Masuhiro Nishikawa, Kazuei Igarashi and Hiroshi Kobayashi2

Faculty of Pharmaceutical Sciences, Chiba University Chiba, Chiba 260

2To whom all correspondence should be addressed

Both Escherichia coli and Streptococcus faecalis grew in an alkaline medium containing 100 µM carbonyl cyanide m-chlorophenylhydrazone (CCCP). Our data suggested that CCCP functioned as a protonophore at a high pH and that the proton-motive force was dissipated almost completely under such conditions. The pH gradient measured with dimethyl-oxazolidine-2,4-dione and acetylsalicylic acid was very small in both bacteria at a high pH above 8, and was not affected significantly by the addition of CCCP. Based on these findings, we propose that the maintenance of neutral cytoplasmic pH is not obligatory for the growth of E. coli and S. faecalis in an alkaline medium.

1This study was supported In part by a Grant-in-Aid for Scientific Research on Priority Areas of Bioenergetics to H. K. from the Ministry of Education, Science and Culture of Japan.


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