J. Biochem, 1995, Vol. 118, No. 6 1104-1107
© 1995 Japanese Biochemical Society
research-article |
A Novel Protein Phosphatase-1 Inhibitory Protein Potentiated by Protein Kinase C. Isolation from Porcine Aorta Media and Characterization1
Division of Chemistry, Graduate School of Science, Hokkaido University Kita-ku, Sapparo, Hokkaido 060
A novel phosphorylation-dependent inhibitory protein (IP) of porcine aorta myosin light chain phosphatase (PA-MLCP) was purified to homogeneity from porcine aorta media. The molecular mass of IP was 20 kDa. IP phosphorylated by endogenous potentiating kinase (IP-K) inhibited not only PA-MLCP activity, but also that of the catalytic subunit of protein phosphatase-1. The amino acid sequence of a peptide derived from IP phosphorylated with IP-K, RHARVT*VK, shared one of the consensus sequences phosphorylatable by protein kinase C (PKC), where T* was phosphorylated. IP was phosphorylated by PKC and the phosphorylated product inhibited PA-MLCP as strongly as IP phosphorylated with IP-K.
1 This work was supported by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan, and a Special Grant-in-Aid for the Promotion of Education and Science in Hokkaido University provided by the Ministry of Education, Science and Culture.
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|
|
|
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|
|
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|
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|
|
|
|
 |
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
 |
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
H. Wang, M. Eto, W. D. Steers, A. P. Somlyo, and A. V. Somlyo RhoA-mediated Ca2+ Sensitization in Erectile Function J. Biol. Chem., August 16, 2002; 277(34): 30614 - 30621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Somlyo New Roads Leading to Ca2+ Sensitization Circ. Res., July 26, 2002; 91(2): 83 - 84. [Full Text] [PDF]
|
|
|
|
|
|
Q.-R. Liu, P.-W. Zhang, Q. Zhen, D. Walther, X.-B. Wang, and G. R. Uhl KEPI, a PKC-dependent Protein Phosphatase 1 Inhibitor Regulated by Morphine J. Biol. Chem., April 5, 2002; 277(15): 13312 - 13320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. V. Brozovich Myosin Light Chain Phosphatase: It Gets Around Circ. Res., March 22, 2002; 90(5): 500 - 502. [Full Text] [PDF]
|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
S. Sakurada, H. Okamoto, N. Takuwa, N. Sugimoto, and Y. Takuwa Rho activation in excitatory agonist-stimulated vascular smooth muscle Am J Physiol Cell Physiol, August 1, 2001; 281(2): C571 - C578. [Abstract] [Full Text] [PDF]
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|
|
|
|
|
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|
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|
 |
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 Y. Watanabe, M. Ito, Y. Kataoka, H. Wada, M. Koyama, J. Feng, H. Shiku, and M. Nishikawa Protein kinase C-catalyzed phosphorylation of an inhibitory phosphoprotein of myosin phosphatase is involved in human platelet secretion Blood, June 15, 2001; 97(12): 3798 - 3805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
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|
|
|
|
 |
|
 T. Kandabashi, H. Shimokawa, K. Miyata, I. Kunihiro, Y. Kawano, Y. Fukata, T. Higo, K. Egashira, S. Takahashi, K. Kaibuchi, et al. Inhibition of Myosin Phosphatase by Upregulated Rho-Kinase Plays a Key Role for Coronary Artery Spasm in a Porcine Model With Interleukin-1{beta} Circulation, March 21, 2000; 101(11): 1319 - 1323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. SOLWAY What Makes the Airways Contract Abnormally? Is It Inflammation? Am. J. Respir. Crit. Care Med., March 1, 2000; 161(3): S164 - 167. [Full Text] [PDF]
|
|
|
|
|
|
A. P Somlyo and A. V Somlyo Signal transduction by G-proteins, Rho-kinase and protein phosphatase to smooth muscle and non-muscle myosin II J. Physiol., January 15, 2000; 522(2): 177 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
T Kitazawa, N Takizawa, M Ikebe, and M Eto Reconstitution of protein kinase C-induced contractile Ca2+ sensitization in Triton X-100-demembranated rabbit arterial smooth muscle J. Physiol., October 1, 1999; 520(1): 139 - 152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shimokawa, M. Seto, N. Katsumata, M. Amano, T. Kozai, T. Yamawaki, K. Kuwata, T. Kandabashi, K. Egashira, I. Ikegaki, et al. Rho-kinase-mediated pathway induces enhanced myosin light chain phosphorylations in a swine model of coronary artery spasm Cardiovasc Res, September 1, 1999; 43(4): 1029 - 1039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L Li, M Eto, M R Lee, F Morita, M Yazawa, and T Kitazawa Possible involvement of the novel CPI-17 protein in protein kinase C signal transduction of rabbit arterial smooth muscle J. Physiol., May 1, 1998; 508(3): 871 - 881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Katsumata, H. Shimokawa, M. Seto, T. Kozai, T. Yamawaki, K. Kuwata, K. Egashira, I. Ikegaki, T. Asano, Y. Sasaki, et al. Enhanced Myosin Light Chain Phosphorylations as a Central Mechanism for Coronary Artery Spasm in a Swine Model With Interleukin-1฿ Circulation, December 16, 1997; 96(12): 4357 - 4363. [Abstract] [Full Text]
|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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