J. Biochem, 1996, Vol. 120, No. 4 797-802
© 1996 Japanese Biochemical Society
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Structural Organization of the rae28 Gene, a Putative Murine Homologue of the Drosophila polyhomeotic Gene1
*Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University 3–1 Yamadaoku, Suita 565
Laboratory of Animal Genetics, School of Agricultural Sciences, Nagoya University Chikusa-ku, Nagoya 464–01
Mitsubishi Kasei Institute of Life Sciences Machida, Tokyo 194
2To whom correspondence should be addressed. Phone: +81.6.879.8324, Fax: +81-6-879-8326
A putative murine homologue of the Drosophila polyhomeotic gene, named rae28, has been isolated from a genomic library of 129/SV mouse and its structural organization has been analyzed. rae28 is a single gene of approximately 22 kb long and consists of 15 exons. Its 5'-flanking region lacks typical transcriptional regulatory sequences, such as TATA and CCAAT boxes, but contains GC-rich sequences and seven putative binding sites for a transcription factor, Sp1. One major transcription start point has been determined. The overall exon-intron organization suggested that three different Rae28 mRNAs are generated through alternative splicing. Furthermore, the rae28 gene has been located on the R-positive F3 band of mouse chromosome 6 by the direct R-banding fluorescence in situ hybridization methods.
1This work was supported by Grants-in-Aid for Scientific Research (04454170 and 06807014), and a Grant-in-Aid for Creative Basic Research from the Ministry of Education, Science, Sports and Culture of Japan. This work was also supported by a Grant-in- Aid from the Japan Medical Association and one from the Uehara Memorial Foundation. All the nucleotide sequences reported herein have been deposited in the DDBJ DNA database under accession No. D78515 [GenBank] .