J. Biochem, 1996, Vol. 120, No. 4 813-819
© 1996 Japanese Biochemical Society
other |
Structure and Intracellular Localization of Mouse ADP-Ribosylation Factors Type 1 to Type 6 (ARF1–ARF6)1
,2
,4
*Institute of Biological Sciences Tsukuba, Ibaraki 305
Institute of Applied Biochemistry, University of Tsukuba Tsukuba, Ibaraki 305
Gene Experiment Center, University of Tsukuba Tsukuba, Ibaraki 305
4To whom correspondence should be addressed at: Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305. Tel: +81-298-53-6356, Fax: +81-298-53-6006, E-mail: kazunaka{at}sakura.cc.tsukuba.ac.jp
ADP-ribosylation factors (ARFs) are a family of small GTP-binding proteins that are proposed to be involved in the formation of coated transport vesicles. Although six ARF sequences have been reported in mammals to date, it has been unclear how many ARF members are present in a single organism. In this study, we provide the first direct evidence by cDNA cloning for the presence of all six ARF members in mouse. These proteins are highly conserved across mammalian species and Northern blot analysis revealed that mRNAs for all the members were expressed ubiquitously. Transfection of cells with epitope-tagged ARFs revealed that ARFs 1–3 displayed a perinuclear Golgi localization, while ARFs 4–6 appeared to be widely dispersed throughout the cytoplasm. These results suggest that although all the ARF proteins play fundamental and critical roles in cellular function, they are involved in different vesicular transport processes.
1This study was supported in part by grants from the Ministry of Education, Science, Sports and Culture of Japan, the University of Tsukuba Research Project, the Special Research Project on Circulation Biosystem in the University of Tsukuba, the Saneyoshi Scholarship Foundation, the Ciba-Geigy Foundation (Japan) for the Promotion of Science, the Asahi Glass Foundation, Sankyo Co., and Ono Pharmaceutical Co.
2The first two authors contributed equally to this study.
3Present address: Howard Hughes Medical Institute and Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9050, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Y. Dejgaard, A. Murshid, A. Erman, O. Kizilay, D. Verbich, R. Lodge, K. Dejgaard, T. B. N. Ly-Hartig, R. Pepperkok, J. C. Simpson, et al. Rab18 and Rab43 have key roles in ER-Golgi trafficking J. Cell Sci., August 15, 2008; 121(16): 2768 - 2781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Shiba, H. Koga, H.-W. Shin, M. Kawasaki, R. Kato, K. Nakayama, and S. Wakatsuki Structural basis for Rab11-dependent membrane recruitment of a family of Rab11-interacting protein 3 (FIP3)/Arfophilin-1 PNAS, October 17, 2006; 103(42): 15416 - 15421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fujimoto, H. Takatsu, and H. Ohno CMRF-35-like molecule-5 constitutes novel paired receptors, with CMRF-35-like molecule-1, to transduce activation signal upon association with FcR{gamma} Int. Immunol., October 1, 2006; 18(10): 1499 - 1508. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hatsuzawa, T. Tamura, H. Hashimoto, H. Hashimoto, S. Yokoya, M. Miura, H. Nagaya, and I. Wada Involvement of Syntaxin 18, an Endoplasmic Reticulum (ER)-localized SNARE Protein, in ER-mediated Phagocytosis Mol. Biol. Cell, September 1, 2006; 17(9): 3964 - 3977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Lay, B. L. Grosshans, H. Heid, K. Gorgas, and W. W. Just Binding and Functions of ADP-ribosylation Factor on Mammalian and Yeast Peroxisomes J. Biol. Chem., October 14, 2005; 280(41): 34489 - 34499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-W. Shin, H. Kobayashi, M. Kitamura, S. Waguri, T. Suganuma, Y. Uchiyama, and K. Nakayama Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking J. Cell Sci., September 1, 2005; 118(17): 4039 - 4048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. LI, W. G. KELLY, J. M. LOGSDON JR, A. M. SCHURKO, B. D. HARFE, K. L. HILL-HARFE, and R. A. KAHN Functional genomic analysis of the ADP-ribosylation factor family of GTPases: phylogeny among diverse eukaryotes and function in C. elegans FASEB J, December 1, 2004; 18(15): 1834 - 1850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. El Annan, D. Brown, S. Breton, S. Bourgoin, D. A. Ausiello, and V. Marshansky Differential expression and targeting of endogenous Arf1 and Arf6 small GTPases in kidney epithelial cells in situ Am J Physiol Cell Physiol, April 1, 2004; 286(4): C768 - C778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Matsukawa, K. Nakayama, T. Nagao, H. Ichijo, and T. Urushidani Role of ADP-ribosylation Factor 6 (ARF6) in Gastric Acid Secretion J. Biol. Chem., September 19, 2003; 278(38): 36470 - 36475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mitchell, D. N. Robertson, P. J. Holland, D. Collins, E. M. Lutz, and M. S. Johnson ADP-ribosylation Factor-dependent Phospholipase D Activation by the M3 Muscarinic Receptor J. Biol. Chem., September 5, 2003; 278(36): 33818 - 33830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mansour, S. Y. Lee, and B. Pohajdak The N-terminal Coiled Coil Domain of the Cytohesin/ARNO Family of Guanine Nucleotide Exchange Factors Interacts with the Scaffolding Protein CASP J. Biol. Chem., August 23, 2002; 277(35): 32302 - 32309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Shinotsuka, Y. Yoshida, K. Kawamoto, H. Takatsu, and K. Nakayama Overexpression of an ADP-ribosylation Factor-Guanine Nucleotide Exchange Factor, BIG2, Uncouples Brefeldin A-induced Adaptor Protein-1 Coat Dissociation and Membrane Tubulation J. Biol. Chem., March 8, 2002; 277(11): 9468 - 9473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Mazaki, S. Hashimoto, K. Okawa, A. Tsubouchi, K. Nakamura, R. Yagi, H. Yano, A. Kondo, A. Iwamatsu, A. Mizoguchi, et al. An ADP-Ribosylation Factor GTPase-activating Protein Git2-short/KIAA0148 Is Involved in Subcellular Localization of Paxillin and Actin Cytoskeletal Organization Mol. Biol. Cell, March 1, 2001; 12(3): 645 - 662. [Abstract] [Full Text]
|
|
|
|
|
|
C. A. Millar, K. A. Powell, G. R. X. Hickson, M.-F. Bader, and G. W. Gould Evidence for a Role for ADP-ribosylation Factor 6 in Insulin-stimulated Glucose Transporter-4 (GLUT4) Trafficking in 3T3-L1 Adipocytes J. Biol. Chem., June 18, 1999; 274(25): 17619 - 17625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Passreiter, M. Anton, D. Lay, R. Frank, C. Harter, F. T. Wieland, K. Gorgas, and W. W. Just Peroxisome Biogenesis: Involvement of ARF and Coatomer J. Cell Biol., April 20, 1998; 141(2): 373 - 383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Z. Yang, H. Heimberg, C. D'Souza-Schorey, M. M. Mueckler, and P. D. Stahl Subcellular Distribution and Differential Expression of Endogenous ADP-ribosylation Factor 6 in Mammalian Cells J. Biol. Chem., February 13, 1998; 273(7): 4006 - 4011. [Abstract] [Full Text] [PDF]
|
|