Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Ishiwata, S.
Right arrow Articles by Mizugaki, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishiwata, S.
Right arrow Articles by Mizugaki, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 2001, Vol. 129, No. 1 13-17
© 2001 Japanese Biochemical Society

other

Increased Expression of Queuosine Synthesizing Enzyme, tRNA-Guanine Transglycosylase, and Queuosine Levels in tRNA of Leukemic Cells

Shunji Ishiwata*, Jun Katayama*, Hiroki Shindo*, Yasuko Ozawa*, Kunihiko Itoh{dagger} and Michinao Mizugaki*,1

*Department of Pharmaceutical Sciences, Tohoku University Hospital 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574
{dagger}Department of Pharmaceutical Sciences, Akita University Hospital 1-1 Hondo, Akita 010-8543

1To whom correspondence should be addressed. Phone: +81-22-717-7525, Fax: +81-22-717-7545, E-mail: mizugaki{at}mail.cc.tohoku.ac.jp

Queuosine is a modified nucleoside located at the first position of the tRNA anticodon, which is synthesized by tRNA-guanine transglycosylase (TGT). Although the levels of queuosine in cancer cells have been reported to be lower than those in normal cells, the expression levels of TGT remain to be determined. We determined the expression levels of a subunit of TGT (TGT60KD). Contrary of our expectations, the results revealed higher levels of expression of TGT60KD than that in normal cells, and the level of queuosine in the tRNA fraction corresponded with that of TGT60KD expression. These results suggest the possibilities that the expression levels of TGT60KD regulate TGT activity and the levels of queuosine, and that TGT60KD plays significant roles in carcinogenesis. To our knowledge, this is a first report of increased expression levels of TGT60KD in human cancer cells.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Ibba and C. Francklyn
Turning tRNA upside down: When aminoacylation is not a prerequisite to protein synthesis
PNAS, May 18, 2004; 101(20): 7493 - 7494.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.