J. Biochem, 2003, Vol. 133, No. 4 405-413
© 2003 Japanese Biochemical Society
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Genome Structure and Differential Expression of Two Isoforms of a Novel PDZ-Containing Myosin (MysPDZ) (Myo18A)
1 Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University, Seiryo-machi, Aoba-ku, Sendai 980-8575; 2 Department of Molecular and Cellular Biology, Faculty of Science, Niigata University, Niigata 950-2181; and 3 Development and Differentiation Laboratory, Developmental Biology Department, Insect and Animal Sciences Division, National Institute of Agrobiological Sciences, Ikenodai 2, Tukuba, Ibaraki 305-8602
We previously cloned a gene for a novel myosin (called MysPDZ) containing a PDZ-domain from bone marrow stromal cells. This new myosin is found in humans and classified as one of the class XVIII myosins (Myo18A). Here, we report the hematopoietic cell-specific splicing isoform (MysPDZß) in addition to the previously reported isoform (MysPDZ
). Combined with mouse genome sequence data, the overall genome structure and generation of the two spliced isoforms are deduced. The MysPDZß protein lacks a PDZ-domain in the N-terminal region. Studies of the subcellular localization of the two spliced isoforms indicated that MysPDZ containing the PDZ domain co-localizes with the ER-Golgi complex, while MysPDZß, which lacks the PDZ domain, localizes diffusely in the cytoplasm. These results suggest that the isoforms differ in their subcellular localization and may have different functions in membrane ruffling and membrane traffic pathways. The PDZ-containing spliced isoform (MysPDZ) is not expressed in bone marrow hematopoietic cells, whereas MysPDZß lacking the PDZ is specifically expressed in most hematopoietic cells. It is noted that neither isoform is expressed in red blood cells. Interestingly, MysPDZ was detected in mature but not in immature macrophages, and its level increased after the induction of differentiation of M1 cells, suggesting a functional role of PDZ-containing myosin in macrophages.
+ To whom correspondence should be addressed. Tel: +81-22-717-8483, Fax: +81-22-717-8488, E-mail: mobinata{at}idac.tohoku.ac.jp
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