Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Kashima, Y.
Right arrow Articles by Ishikawa, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kashima, Y.
Right arrow Articles by Ishikawa, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 2003, Vol. 134, No. 1 25-29
© 2003 Japanese Biochemical Society

BIOCHEMISTRY

Alkyl Hydroperoxide Reductase Dependent on Thioredoxin-Like Protein from Pyrococcus horikoshii

Yasuhiro Kashima and Kazuhiko Ishikawa+

Special Division of Human Life Technology, National Institute of Advanced Industrial Science and Technology (AIST Kansai) 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577

Pyrococcus horikoshii is an obligate anaerobic hyperthermophilic archaeon. In P. horikoshii cells, a hydroperoxide reductase homologue ORF (PH1217) was found to be induced by oxygen. The recombinant protein, which was expressed in E. coli under aerobic conditions, exhibited no activity. However, the recombinant protein prepared under semi-anaerobic conditions exhibited alkyl hydroperoxide reductase activity. Furthermore, it was clarified that it was coupled with the thioredoxin-like system in P. horikoshii. Western blot analysis revealed that the protein was induced by oxygen and hydrogen peroxide. This protein seems to be sensitive to oxygen but forms a thioredoxin-dependent system to eliminate reactive oxygen species in P. horikoshii.

+ To whom correspondence should be addressed. Tel: +81-727-51-9526, Fax: +81-727-51-9628, E-mail: kazu-ishikawa{at}aist.go.jp


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
M. A. Pysz, S. B. Conners, C. I. Montero, K. R. Shockley, M. R. Johnson, D. E. Ward, and R. M. Kelly
Transcriptional Analysis of Biofilm Formation Processes in the Anaerobic, Hyperthermophilic Bacterium Thermotoga maritima
Appl. Envir. Microbiol., October 1, 2004; 70(10): 6098 - 6112.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
R. Kawakami, H. Sakuraba, S. Kamohara, S. Goda, Y. Kawarabayasi, and T. Ohshima
Oxidative Stress Response in an Anaerobic Hyperthermophilic Archaeon: Presence of a Functional Peroxiredoxin in Pyrococcus horikoshii
J. Biochem., October 1, 2004; 136(4): 541 - 547.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.