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J. Biochem, 2003, Vol. 134, No. 2 219-224
© 2003 Japanese Biochemical Society

BIOTECHNOLOGY

Elevation of Plasma Membrane Permeability on Laser Irradiation of Extracellular Latex Particles

Yukihiro Umebayashi, Yuuichi Miyamoto, Masayoshi Wakita, Akiko Kobayashi and Tsuyoshi Nishisaka*,§

School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Tatsunokuchi, Nomi-gun, Ishikawa 923-1292

In this report, we describe a laser-latex combination system that enables membrane-impermeable molecules to penetrate cell membranes. Laser light (Q-switched Nd:YAG laser, 532.5 nm) was used to irradiate a mixture of commercial latex particles (blue dyed, 1 µm in diameter) and mouse fibrosarcoma (Meth-A) cells. After irradiation, membrane permeability was evaluated by flow cytometric assaying using propidium iodide (PI) and fluorescein diacetate (FDA). The proportion of permeabilized-resealed cells was affected by changes in the light intensity (~780 mW/cm2), the irradiation time (~240 s), and/or the particle concentration (~109 particles/ml). The permeability persisted up to 20 min after light irradiation. Near the sites of individual particles, the permeability of the cell membrane is modified, probably due to localized temperature changes. These results suggest that this laser-induced permeabilization strategy constitutes a new means of delivering exogenous materials into living cells.

* This study was partly supported by a Grant-in-Aid for Exploratory Research (No. 12878167) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

§ To whom correspondence should be addressed. Tel: +81-761-51-1685, Fax: +81-761-51-1685, E-mail: saka{at}jaist.ac.jp


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