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J. Biochem, 2003, Vol. 134, No. 2 231-238
© 2003 Japanese Biochemical Society

MOLECULAR BIOLOGY

Molecular Cloning of a Novel Transmembrane Protein MOLT Expressed by Mature Oligodendrocytes

Hiroyuki Nomoto1,2, Tomoko Yonezawa1, Kouichi Itoh3, Katsuhiko Ono4, Kiyotaka Yamamoto5, Toshitaka Oohashi1, Fumio Shiraga6, Hiroshi Ohtsuki2 and Yoshifumi Ninomiya*,1

Departments of 1 Molecular Biology and Biochemistry and 2 Ophthalmology, Neuroscience and Functional Physiology, Biophysiological Science, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558; 3 Department of Pharmacology, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Research Organization, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613; 4 National Institute for Physiological Sciences, Okazaki, 444-8585; 5 Department of Cell Biology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173-0015; and 6 Department of Ophthalmology, Kagawa Medical University, 1750-1 Ikenobe Miki-cho, Kita-Gun, Kagawa 761-0793

A novel oligodendrocyte (OL)-specific cDNA was isolated from brain capillary endothelial cells and characterized. The cDNA encodes a protein of 1099 amino acids that contains a signal peptide and a transmembrane domain. The protein was expressed in mature OLs in vivo and in vitro cell cultures and was thus designated as mature OL transmembrane protein (MOLT). RT-PCR analysis showed that MOLT mRNA was expressed in brain, lung, pancreas, and testis. A polyclonal antibody raised against a part of the mouse MOLT reacted specifically with multipolar OLs possessing radially oriented processes that penetrated into the gray matter. More cells were detected in the white matter, and these had longitudinally oriented processes. In a rat OL lineage culture system, oligodendrocyte precursor cells did not initially produce MOLT mRNA and protein, but when they begun to differentiate into mature OLs, they started expressing MOLT. Consequently, MOLT may function as OLs become mature and may serve as a cell-surface marker for OL differentiation.

* To whom correspondence should be addressed. Tel: +81-86-235-7127, Fax: +81-86-222-7768, E-mail: yoshinin{at}cc.okayama-u.ac.jp


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