J. Biochem, 2004, Vol. 135, No. 1 101-107
© 2004 The Japanese Biochemical Society
BIOCHEMISTRY |
Characterization of Recombinant CEL-I, a GalNAc-Specific C-Type Lectin, Expressed in Escherichia coli Using an Artificial Synthetic Gene
1 Department of Applied Chemistry, Faculty of Engineering, and 2 Division of Biochemistry, Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521; and 3 Metabolic Function Research Group, RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045
CEL-I is a C-type lectin isolated from the Holothuroidea Cucumaria echinata. This lectin shows very high N-acetylgalactosamine-binding specificity. We constructed an artificial gene encoding recombinant CEL-I (rCEL-I) using a combination of synthetic oligonucleotides, and expressed it in Escherichia coli cells. Since the recombinant protein was obtained as inclusion bodies, the latter were solubilized using urea and 2-mercaptoethanol, and the protein was refolded during the purification and dialysis steps. The purified rCEL-I showed comparable hemagglutinating activity to that of native CEL-I at relatively high Ca2+-concentrations, whereas it was weaker at lower Ca2+-concentrations due to decreased Ca2+-binding affinity. rCEL-I exhibited similar carbohydrate-binding specificity to native CEL-I, including strong GalNAc-binding specificity, as examined by hemagglutination inhibition assay. Comparison of the far UV-CD spectra of recombinant and native CEL-I revealed that the two proteins undergo a similar conformational change upon binding of Ca2+. Single crystals of rCEL-I were also obtained under the same conditions as those used for the native protein, suggesting that they have similar tertiary structures. Although native CEL-I exhibited strong cytotoxicity toward cultured cells, rCEL-I showed low cytotoxicity. These results indicate that rCEL-I has a tertiary structure and carbohydrate-binding specificity similar to those of native CEL-I. Howeger, there is a subtle difference in the properties between the two proteins probably due to the additional methionine residue at the N-terminus of rCEL-I.
* To whom correspondence should be addressed. Fax: +81-95-819-2684, E-mail: thata{at}net.nagasaki-u.ac.jp
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Yamanishi, T. Hatakeyama, K. Yamaguchi, and T. Oda CEL-I, an N-Acetylgalactosamine (GalNAc)-Specific C-Type Lectin, Induces Nitric Oxide Production in RAW264.7 Mouse Macrophage Cell Line J. Biochem., August 1, 2009; 146(2): 209 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamanishi, Y. Yamamoto, T. Hatakeyama, K. Yamaguchi, and T. Oda CEL-I, an Invertebrate N-Acetylgalactosamine-specific C-Type Lectin, Induces TNF-{alpha} and G-CSF Production by Mouse Macrophage Cell Line RAW264.7 Cells J. Biochem., November 1, 2007; 142(5): 587 - 595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kuramoto, H. Uzuyama, T. Hatakeyama, T. Tamura, T. Nakashima, K. Yamaguchi, and T. Oda Cytotoxicity of a GalNAc-Specific C-Type Lectin CEL-I toward Various Cell Lines J. Biochem., January 1, 2005; 137(1): 41 - 50. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Sugawara, M. Kusunoki, G. Kurisu, T. Fujimoto, H. Aoyagi, and T. Hatakeyama Characteristic Recognition of N-Acetylgalactosamine by an Invertebrate C-type Lectin, CEL-I, Revealed by X-ray Crystallographic Analysis J. Biol. Chem., October 22, 2004; 279(43): 45219 - 45225. [Abstract] [Full Text] [PDF]
|
|