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J. Biochem, 2004, Vol. 135, No. 2 165-169
© 2004 The Japanese Biochemical Society

MOLECULAR BIOLOGY

Regulatory Elements Directing Gut Expression of the GATA6 Gene during Mouse Early Development

Ge-Hong Sun-Wada, Yoichi Kamei, Yoh Wada and Masamitsu Futai*

Division of Biological Sciences, The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047

The GATA6 transcription factor, as well as GATA4 and GATA5, is expressed in a variety of mammalian tissues including the precardiac mesoderm and endoderm, and gut-related organs. Genetic studies have also implicated GATA factors as important regulators of gut endoderm development. Previously, we identified the promoter and a cardiac-specific enhancer of mouse GATA6 [Sun-Wada et al. (2000) J. Biochem. 127, 703–709], however, little is known about the regulatory elements that govern GATA6 expression in the primitive gut. Here, we identified a distal enhancer of the GATA6 gene directing expression in the gut by creating transgenic mice. A sequence of approximately 200 bp between –8.0 kb and –7.8 kb contains element(s) that enhance transcription in the gut during embryonic development, when linked to the hsp68 promoter/lacZ fusion gene. Our results also show that GATA6 expression is controlled by multiple regulatory regions including cardiac-specific and gut-specific enhancers.

* To whom Correspondence should be addressed. Tel: +81-6-6879-8480, Fax: +81-6-6875-5724, E-mail: m-futai{at}sanken.osaka-u.ac.jp


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