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J. Biochem, 2004, Vol. 136, No. 4 421-425
© 2004 The Japanese Biochemical Society

BIOTECHNOLOGY

Establishment of Stable hFis1 Knockdown Cells with an siRNA Expression Vector

Ryoichi Arai1,3,*, Kaori Ito1,*, Motoaki Wakiyama1, Eiko Matsumoto1, Ayako Sakamoto1, Yuuka Etou1, Makiko Otsuki1, Mio Inoue1, Yoshihide Hayashizaki2, Makoto Miyagishi5,6, Kazunari Taira5,6, Mikako Shirouzu1,3 and Shigeyuki Yokoyama1,3,4,7,§

1 Protein Research Group and 2 Genome Exploration Research Group, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045; 3 Structurome Research Group and 4 Structural and Molecular Biology Laboratory, RIKEN Harima Institute at SPring-8, 1-1-1 Kouto, Mikazuki-cho, Sayo, Hyogo 679-5148; 5 Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656; 6 Gene Function Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba 305-8562; and 7 Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033

Yeast Fis1p participates in mitochondrial fission, together with Dnm1p and Mdv1p. Recently, human Fis1 (hFis1) was reported to be involved in mitochondrial fission, together with Drp1. We established stable transformants with an hFis1 siRNA expression vector. In the stable hFis1 knockdown cells, hFis1 expression was suppressed to approximately 10%, and mitochondrial fission, induced by cisplatin treatment, was delayed. In addition, mouse Fis1 (mFis1) expression promoted mitochondrial fission and cell death in the hFis1 knockdown cells, suggesting that mFis1 complements the function of hFis1. These hFis1 siRNA expression vectors may be useful for studying the molecular function of mammalian Fis1.

* These authors contributed equally to this work.

§ To whom correspondence should be addressed. Tel: +81-45-503-9196, Fax: +81-45-503-9195, E-mail: yokoyama{at}biochem.s.u-tokyo.ac.jp


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