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Journal of Biochemistry 2005 138(4):391-397; doi:10.1093/jb/mvi134
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© 2005 The Japanese Biochemical Society

Regular Paper

N-Acetyltransferase Mpr1 Confers Freeze Tolerance on Saccharomyces cerevisiae by Reducing Reactive Oxygen Species

Xiaoyi Du and Hiroshi Takagi*

Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjojima, Matsuoka-cho, Fukui 910-1195

* To whom correspondence should be addressed. Tel: +81-776-61-6000, Fax: +81-776-61-6015, E-mail: hiro{at}fpu.ac.jp

N-Acetyltransferase Mpr1 of Saccharomyces cerevisiae can reduce intracellular oxidation levels and protect yeast cells under oxidative stress. We found that yeast cells exhibited increased levels of reactive oxygen species during freezing and thawing. Gene disruption and expression experiments indicated that Mpr1 protects yeast cells from freezing stress by reducing the intracellular levels of reactive oxygen species. The combination of Mpr1 and L-proline could further enhance the resistance to freezing stress. Hence, Mpr1 as well as L-proline has promising potential for the breeding of novel freeze-tolerant yeast strains.


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