Skip Navigation

Journal of Biochemistry 2005 138(6):763-772; doi:10.1093/jb/mvi168
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Torisu, K.
Right arrow Articles by Nakabeppu, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Torisu, K.
Right arrow Articles by Nakabeppu, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2005 The Japanese Biochemical Society.

Regular Paper

Hematopoietic Tissue–Specific Expression of Mouse Neil3 for Endonuclease VIII–Like Protein

Kumiko Torisu, Daisuke Tsuchimoto*, Yoshinori Ohnishi and Yusaku Nakabeppu

Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi Higashi-ku, Fukuoka 812-8582

* To whom correspondence should be addressed. Tel: +81-92-642-6802, Fax: +81-92-64-6804, E-mail: daisuke{at}bioreg.kyushu-u.ac.jp

We cloned cDNA and genomic DNA containing exon 1 of mouse Neil3. Neil3 spans 52.4 kb and consists of 10 exons. Northern blot analysis revealed that Neil3 mRNA was selectively expressed in thymus, spleen and bone marrow. High levels of Neil3 mRNA were also detected in various mouse B cell lines by RT-PCR. Immunofluorescence microscopy using anti-NEIL3 revealed that recombinant mouse NEIL3 is localized in the nuclei. In mouse splenocytes, the level of Neil3 mRNA significantly increased after mitogen stimulation in vitro. We established NEIL3-null mice, which are viable and fertile. We found candidate sequences for NEIL3 orthologues in a DNA database from dog and zebrafish in addition to human and mouse, but not invertebrates. NEIL3 may function exclusively in vertebrates, such as mammals.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GENES CELLSHome page
M. Takao, Y. Oohata, K. Kitadokoro, K. Kobayashi, S. Iwai, A. Yasui, S. Yonei, and Q.-M. Zhang
Human Nei-like protein NEIL3 has AP lyase activity specific for single-stranded DNA and confers oxidative stress resistance in Escherichia coli mutant.
Genes Cells, February 1, 2009; 14(2): 261 - 270.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.