Skip Navigation

Journal of Biochemistry 2006 139(5):837-846; doi:10.1093/jb/mvj095
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Gopinath, S. C.B.
Right arrow Articles by Kumar, P. K.R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gopinath, S. C.B.
Right arrow Articles by Kumar, P. K.R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2006 The Japanese Biochemical Society.

Regular Paper

An Efficient RNA Aptamer against Human Influenza B Virus Hemagglutinin

Subash C.B. Gopinath, Yuriko Sakamaki, Kazunori Kawasaki and Penmetcha K.R. Kumar*

Functional Nucleic Acids Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1 Higashi, Tsukuba, Ibaraki 305-8566

* To whom correspondence should be addressed. Tel: +81-298-61-6085, Fax: +81-298-61-6095, E-mail: pkr-kumar{at}aist.go.jp

Aptamers are known for their higher discriminating ability between closely related molecules and their requirement for only a small region for binding, as compared to an antibody. In the present studies, we have isolated a specific RNA aptamer against the influenza virus B/Johannesburg/05/1999 by an in vitro selection procedure. The aptamer bound efficiently to the HA of influenza B and required 5 mM MgCl2 ion for its recognition. The aptamer not only distinguished HA derived from the influenza A virus, but also inhibited HA-mediated membrane fusion.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Acta Biochim Biophys SinHome page
C. Wang, G. Yang, Z. Luo, and H. Ding
In vitro selection of high-affinity DNA aptamers for streptavidin
Acta Biochim Biophys Sin, April 1, 2009; 41(4): 335 - 340.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
W. James
Aptamers in the virologists' toolkit
J. Gen. Virol., February 1, 2007; 88(2): 351 - 364.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
S. C.B. Gopinath, D. Balasundaresan, J. Akitomi, and H. Mizuno
An RNA Aptamer That Discriminates Bovine Factor IX from Human Factor IX
J. Biochem., November 1, 2006; 140(5): 667 - 676.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.