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Journal of Biochemistry Advance Access originally published online on October 28, 2006
Journal of Biochemistry 2006 140(6):813-823; doi:10.1093/jb/mvj215
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© 2006 The Japanese Biochemical Society.

ARTICLE

Cloning and Sequencing of cDNA and Genomic DNA Encoding PDM Phosphatase of Fusarium moniliforme

Hiroshi Yoshida1,2,*, Mari Iizuka1, Takao Narita2, Naoko Norioka3 and Shigemi Norioka3,{dagger}

1 Department of Chemistry, Faculty of Medicine, Shimane University, 89-1 En-ya-cho, Izumo 693-8501; 2 Department of Chemistry, Graduate School of Science, Tohoku University, Aramaki Aza-Aoba, Aoba-ku, Sendai 980-8578; and 3 Division of Protein Chemistry, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita 565-0871

*To whom correspondence should be sent at the present address: 1-15-4-1703 Senda-machi, Naka-ku, Hiroshima 730-0052. Tel/Fax: +81-82-242-5417, E-mail: yoshidah{at}withe.ne.jp


  Abstract

PDM phosphatase was purified approximately 500-fold through six steps from the extract of dried powder of the culture filtrate of Fusarium moniliforme. The purified preparation appeared homogeneous on SDS-PAGE although the protein band was broad. Amino acid sequence information was collected on tryptic peptides from this preparation. cDNA cloning was carried out based on the information. A full-length cDNA was obtained and sequenced. The sequence had an open reading frame of 651 amino acid residues with a molecular mass of 69,988 Da. Cloning and sequencing of the genomic DNA corresponding to the cDNA was also conducted. The deduced amino acid sequence could account for many but not all of the tryptic peptides, suggesting presence of contaminant protein(s). SDS-PAGE analysis after chemical deglycosylation showed two proteins with molecular masses of 58 and 68 kDa. This implied that the 58 kDa protein had been copurified with PDM phosphatase. Homology search showed that PDM phosphatase belongs to the purple acid phosphatase family, which is widely distributed in the biosphere. Sequence data of fungal purple acid phosphatases were collected from the database. Processing of the data revealed presence of two types, whose evolutionary relationships were discussed.

{dagger} Present address: 4-9-1 Kita-kasugaoka, Ibaraki 567-0048.


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