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Journal of Biochemistry Advance Access originally published online on November 7, 2006
Journal of Biochemistry 2006 140(6):851-859; doi:10.1093/jb/mvj218
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© 2006 The Japanese Biochemical Society.

ARTICLE

Inhibitory Activity of 1-Farnesylpyridinium on the Spatial Control over the Assembly of Cell Wall Polysaccharides in Schizosaccharomyces pombe

Masahiro Hamada1,*, Ikumi Ohata1, Ken-ichi Fujita1, Yoshinosuke Usuki1, Akira Ogita2, Junpei Ishiguro3 and Toshio Tanaka1,{dagger}

1 Graduate School of Science and 2 Research Center for Urban Health and Sports, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585; and 3 Department of Biology, Faculty of Science and Engineering, Konan University, Okamoto 8-9-1, Kobe 658-8501

{dagger} To whom correspondence should be addressed. Phone: +81-6-6605-3163, Fax: +81-6-6605-3164, E-mail: tanakato{at}sci.osaka-cu.ac.jp


  Abstract

The modes of actions of 1-farnesylpyridinium (FPy) on yeast cell growth were investigated on the basis of its effects on cell cycle progression, morphogenesis and the related events for construction of cell wall architecture in Schizosacchromyces pombe. FPy predominantly inhibited the growth of the yeast cells after various cycles of cell division so that cells were arrested at the phase of separation into daughter cells accompanying morphological changes to swollen spherical cells at 24 h of incubation. FPy-treated cells were osmotically stable but were susceptible to the lytic action of (1, 3) ß-D-glucanases, and characterized by serious damages to the cell wall architecture as represented by a rough and irregular surface outlook. The isolated cell wall fraction gave a similar hexose composition with or without FPy treatment, suggesting that FPy did not inhibit the synthesis of each cell wall polysaccharide. FPy was permissive for the extracellular accumulation of amorphous cell wall materials and septum development in protoplasts, but absolutely interfered with the following morphogenetic process for construction of the rod-shaped cell wall architecture. Our results suggest the inhibitory activity of FPy on the spatial control over the assembly of cell wall polysaccharides.

* Present address: Research Center, Earth Biochemical Co., Ltd., 923 Kagasuno, Kawauchicho, Tokushima 771-0130.


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