Skip Navigation


Journal of Biochemistry Advance Access originally published online on July 23, 2007
Journal of Biochemistry 2007 142(3):377-381; doi:10.1093/jb/mvm144
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
142/3/377    most recent
mvm144v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Miyagi, Y.
Right arrow Articles by Sagami, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyagi, Y.
Right arrow Articles by Sagami, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Japanese Biochemical Society.

Human Geranylgeranyl Diphosphate Synthase is an Octamer in Solution

Yukino Miyagi, Yoshihiro Matsumura and Hiroshi Sagami*

Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1, Katahira, Aoba-ku, Sendai 980-8577, Japan

*To whom correspondence should be addressed. Tel: +81-22-217-5622, Fax: +81-22-217-5620, E-mail: yasagami{at}tagen.tohoku.ac.jp

Received May 9, 2007; Accepted June 17, 2007


  Abstract

A recombinant geranylgeranyl diphosphate synthase (GGPS) was analysed to be a mixture of octamer, hexamer and dimer by gel filtration using a Superdex 200 column followed by the blue native polyacrylamide gel electrophoresis. The hexamer and dimer were each converted to an octamer by treating with dithiothreitol (DTT). When the recombinant GGPS was preliminarily treated with DTT and similarly analysed, octamer was predominantly detected with a trace amount of hexamer. The octameric form of GGPS was also supported by the cross-linking experiments with bis(sulfosuccinimidyl) suberate. The GGPS in an octameric form was active with a combination of farnesyl diphosphate and [1-14C]isopentenyl diphosphate. These results indicate that the active form of GGPS in the solution is an octamer rather than hexamer or dimer.

Key Words: cross-linking, geranylgeranyl diphosphate synthase, isoprenylation, prenyltransferase, structure and activity

Abbreviations: BS3, bis(sulfosuccinimidyl) suberate; DTT, dithiothreitol; FPS, farnesyl diphosphate synthase; GGPS, geranylgeranyl diphosphate synthase; PAGE, polyacrylamide gel electrophoresis; PP, diphosphate; TCEP, tris(2-carboxyethyl)phosphine


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.