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Journal of Biochemistry Advance Access originally published online on September 18, 2007
Journal of Biochemistry 2007 142(5):613-620; doi:10.1093/jb/mvm176
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© 2007 The Japanese Biochemical Society

BAF53 Interacts with p53 and Functions in p53-mediated p21-gene Transcription

Min Wang, Chunsheng Gu, Tianyang Qi, Wen Tang, Ling Wang, Shuyan Wang and Xianlu Zeng*

Institute of Genetics and Cytology, Northeast Normal University, Changchun, China

*To whom correspondence should be addressed. Tel: +86-431-85099317, Fax: +86-431-85681186, E-mail: zengx779{at}nenu.edu.cn

Received August 8, 2007; Accepted August 19, 2007


  Abstract

BAF53, a component of chromatin remodelling and histone acetyltransferase complexes, has been shown to be essential for cell survival in human cells and plays roles in p53-mediated gene transcription. However, the mechanism concerned in the process needs to be further explored. In this study, we show that BAF53 is involved in the repression of p53-dependent p21-gene transcription by interacting with p53 both in vivo and in vitro. Through electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation analyses, we demonstrate that BAF53 can reduce the p53-binding ability to p21 promoter. By western-blot experiments, we find that BAF53 can decrease p53-Lys382 acetylation, which may be partially responsible for the repression of p53-binding ability. Furthermore, BAF53 represses p21-promoter activity in a BRG1-independent manner. These data contribute to elucidating the molecular mechanisms of BAF53 in regulating p53-mediated gene transcription.

Key Words: BAF53, p53, p21-gene transcription, p53 acetylation

Abbreviations: Arps, actin-related proteins; BAFs, BRG1-associated factors; PREs, p53-response elements; ChIP, chromatin immunoprecipitation; EMSA, electrophoretic mobility shift assay


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