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Journal of Biochemistry Advance Access originally published online on November 16, 2007
Journal of Biochemistry 2008 143(3):311-324; doi:10.1093/jb/mvm223
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© 2007 The Japanese Biochemical Society.

Induction of Cell Adhesion by Galectin-8 and its Target Molecules in Jurkat T-Cells

Hitomi Yamamoto1, Nozomu Nishi1,2, Hiroki Shoji1, Aiko Itoh2, Liang-Hao Lu1, Mitsuomi Hirashima2,3 and Takanori Nakamura1,*

1Department of Endocrinology, Faculty of Medicine, Kagawa University; 2Galpharma Co., Ltd., Kagawa; and 3Department of Immunology and Immunopathology, Faculty of Medicine, Kagawa University, Kagawa, Japan

*To whom correspondence should be addressed. Tel: +81-87-891-2106, Fax: +81-87-891-2108, E-mail: tnaka{at}med.kagawa-u.ac.jp

Received September 3, 2007; Accepted November 6, 2007


  Abstract

We previously showed that tandem-repeat type galectin-8, which has two covalently linked carbohydrate recognition domains (CRDs), induces neutrophil-adhesion through binding to integrin {alpha}M. Here, we analysed the function of galectin-8 in Jurkat T-cells. Galectin-8, as well as tandem-repeat galectin-9, and several multivalent plant lectins, induced Jurkat T-cell adhesion to a culture plate, whereas single-CRD galectins-1 and -3 did not. Galectin-8 also induced the adhesion of peripheral blood leucocytes to human umbilical vein endothelial cells. These results suggest that the di- or multi-valent structure of galectin-8 is essential for the induction of cell adhesion and that this ability exhibits broad specificity for leucocytes. The galectin-8-induced cell adhesion was accompanied by stress fibre formation, which suggests that intracellular signalling is required. We have identified integrin {alpha}4 as one of the candidate target molecules associated with the induction of cell adhesion. Indeed, inhibition of the function of integrin {alpha}4 by treating cells with a blocking-antibody reduced the sensitivity to galectin-8. Also, the phosphorylation of Pyk and ERK1/2, indicators of integrin-mediated signalling, was up-regulated on treatment with galectin-8. Thus, a primary target of galectin-8 must be the sugar chains on members of the integrin family, which are abundantly expressed on the surface of leucocytic cells.

Key Words: adhesion, cytoskeleton arrangement, galectin-8, integrin, leucocyte

Abbreviations: ECM, extracellular matrix; EDTA, ethylenediamine tetra-acetic acid; ERK, extracellular signal-regulated kinase; GST, glutathione S-transferase; MAPK, mitogen-activated protein kinase; Pyk2, proline-rich tyrosine kinase-2; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis


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