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Journal of Biochemistry Advance Access originally published online on January 22, 2008
Journal of Biochemistry 2008 143(4):547-554; doi:10.1093/jb/mvn003
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© 2008 The Japanese Biochemical Society.

Identification of β1,4GalT II as a Target Gene of p53-mediated HeLa Cell Apoptosis

Jin Zhou, Yuanyan Wei, Dan Liu, Xuqing Ge, Fengbiao Zhou, Xiaojing Yun, Jianhai Jiang* and Jianxin Gu

Key Laboratory of Glycoconjuates Research, Ministry of Public Health & Gene Research Center, Shanghai Medical College of Fudan University, Shanghai 200032, People's Republic of China

*To whom correspondence should be addressed. Tel: +86-21-54237660, Fax: +86-21-64164489, E-mail: jianhaijiang{at}fudan.edu.cn

Received October 3, 2007; Accepted December 22, 2007


  Abstract

β1,4-galactosyltransferase II (β1,4GalT II) is one of the enzymes transferring galactose to the terminal N-acetylglucosamine of complex-type N-glycans. Previously, we have reported that β1,4GalT II overexpression increased cisplatin-induced HeLa cell apoptosis. However, the mechanisms of its expression regulation have been rarely investigated. Here, we cloned the 1.8-kb 5'-flanking region of the β1,4GalT II gene and analysed its promoter activity. The transcriptional activity and mRNA expression level of β1,4GalT II were dramatically induced by p53 transcription factor in HeLa cells. In response to DNA damage agent adriamycin, the mRNA expression and promoter activity of β1,4GalT II were significantly up-regulated and the binding of p53 to β1,4GalT II promoter was obviously increased. Furthermore, decreasing the expression of β1,4GalT II using RNA interference inhibited p53-mediated HeLa cell apoptosis induced by adriamycin. Collectively, these results suggested that β1,4GalT II might serve as a target gene of p53 transcription factor during adriamycin-induced HeLa cell apoptosis, which elucidated a new mechanism of p53-mediated cell apoptosis.

Key Words: adriamycin, apoptosis, β1,4-galactosyltransferase II, p53, transcription regulation

Abbreviations: ChIP, chromatin immunoprecipitation; β1, 4GalT II, β1,4-galactosyltransferase II; RCA-I, Ricinus communis agglutinin-I; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HRP, horseradish peroxidase; SDS-PAGE, SDS-poly-acrylamide gel


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