Skip Navigation


Journal of Biochemistry Advance Access originally published online on April 19, 2008
Journal of Biochemistry 2008 144(2):177-186; doi:10.1093/jb/mvn054
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
144/2/177    most recent
mvn054v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Araújo, J. R.
Right arrow Articles by Martel, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Araújo, J. R.
Right arrow Articles by Martel, F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Japanese Biochemical Society

Modulation of Glucose Uptake in a Human Choriocarcinoma Cell Line (BeWo) by Dietary Bioactive Compounds and Drugs of Abuse

João R. Araújo, Pedro Gonçalves and Fátima Martel*

Department of Biochemistry (U38-FCT), Faculty of Medicine, University of Porto, Al. Prof. Hernâni Monteiro, 4200-319 Porto, Portugal

*To whom correspondence should be addressed. Tel: 351 22 551 36 24, Fax: 351 22 551 36 24, E-mail: fmartel{at}med.up.pt

Received February 8, 2008; Accepted April 11, 2008


  Abstract

The aim of this work was to investigate the putative modulation of glucose uptake in trophoblast cells by several dietary compounds and by drugs of abuse. For this, the acute (26 min) and chronic (48 h) effect of these substances on the apical uptake of 3H-2-deoxy-D-glucose (3H-DG) by a human choriocarcinoma cell line (BeWo) was determined. 3H-DG apical uptake by BeWo cells was time dependent, displayed saturable kinetics (Vmax = 1210 ± 29 nmol mg protein–1 6 min–1 and Km = 13.4 ± 0.5 mM) and was insulin-insensitive and cytochalasin B-sensitive (by up to 60%). Acutely, acetaldehyde (30–100 mM), resveratrol, xanthohumol, epigallocatechin-3-gallate (100 µM), chrysin and quercetin (10–100 µM) decreased 3H-DG apical uptake, whereas rutin, catechin (10–100 µM), epicatechin (100 µM) and ethanol (10 mM) increased it. Quercetin and xanthohumol seem to be non-competitive inhibitors of 3H-DG apical uptake, whereas both epigallocatechin-3-gallate and acetaldehyde decreased both the Km and Vmax values. Chronically, rutin and myricetin increased the apical uptake of 3H-DG both isolated (0.1–1 µM) and in combination (both at 1 µM), whereas theophylline (0.1–1 µM) and amphetamine, 3,4-methylenedioxymethamphetamine (0.25–1 µM) and {Delta}9-tetrahydrocannabinol (1 nM) decreased it. In conclusion, 3H-DG apical uptake by BeWo cells is differentially modulated by different compounds present in drinks and by drugs of abuse.

Key Words: BeWo cells, drugs of abuse, glucose uptake, methylxanthines, polyphenols

Abbreviations: DG, 2-deoxy-D-glucose; EGCG, epigallocatechin-3-gallate; GLUT, facilitative glucose family of transporters; IGF-1, insulin growth factor-1; MDMA, 3,4-methylenedioxymetamphetamine; SGLT1, sodium-glucose co-transporter 1; THC, tetrahydrocannabinol


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.