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Journal of Biochemistry Advance Access originally published online on October 30, 2008
Journal of Biochemistry 2009 145(1):51-58; doi:10.1093/jb/mvn146
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© The Authors 2008. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

Establishment and Characterization of Hepatic Stem-like Cell lines from Normal Adult Rat Liver

Mitsuhi Hirata, Kumiko Amano, Ayumi Miyashita, Mayu Yasunaga, Tomoko Nakanishi and Kenzo Sato*

Division of Molecular Biology, School of Life Science, Tottori University Faculty of Medicine, 86 Nishicho, Yonago 683-8503, Japan

*To whom correspondence should be addressed. Tel: 81-859-38-6201, Fax: +81-859-38-6200, E-mail: kensato{at}med.tottori-u.ac.jp

Received September 4, 2008; Accepted October 16, 2008


  Abstract

The liver is a unique organ with the potential to regenerate from injury. Hepatic stem cells contribute to liver regeneration when surviving hepatocytes in injured liver are unable to proliferate. To investigate the mechanism of liver regeneration in vitro, we established hepatic stem cell lines named HY1, HY2 and HY3, derived from a healthy liver of adult rat. HY cells showed an expression pattern similar to oval cells, and efficiently induced hepatic differentiation following sequential treatment with type I collagen, transforming growth factor-β1 (TGF-β1), and hepatocyte growth factor (HGF) or oncostatin M (OSM). These results suggested that HY cells are liver stem cells representing an excellent tool for in vitro studies on liver regeneration.

Key Words: hepatic stem cell, hepatic differentiation, type I collagen, TGF-β1, HGF

Abbreviations: TGF-β1, transforming growth factor-β1; HGF, hepatocyte growth factor; OSM, oncostatin M; ECM, extracellular matrix


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