Skip Navigation


Journal of Biochemistry Advance Access originally published online on October 30, 2008
Journal of Biochemistry 2009 145(1):59-66; doi:10.1093/jb/mvn143
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
145/1/59    most recent
mvn143v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Ujiie, H.
Right arrow Articles by Muto, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ujiie, H.
Right arrow Articles by Muto, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Authors 2008. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

Trans-Translation is Involved in the CcpA-Dependent Tagging and Degradation of TreP in Bacillus subtilis

Hiromi Ujiie1,2, Tomoko Matsutani1, Hisashi Tomatsu1,3, Ai Fujihara1,3, Chisato Ushida1,2,3,4, Yasuhiko Miwa5, Yasutaro Fujita5, Hyouta Himeno1,2,3,4 and Akira Muto1,2,3,4,*

1Department of Biochemistry and Molecular Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036-8561; 2The United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8551; 3Department of Biology, Faculty of Science; 4RNA Research Center, Hirosaki University, Hirosaki 036-8561; and 5Faculty of Biotechnology, Fukuyama University, Fukuyama 729-0292, Japan

*To whom correspondence should be addressed. Tel: +81-172-39-3592; Fax: +81-172-39-3593; E-mail: muto{at}cc.hirosaki-u.ac.jp

Received September 30, 2008; Accepted October 17, 2008


  Abstract

TreP [trehalose-permease (phosphotransferase system (PTS) trehalose-specific enzyme IIBC component)] is one of the target proteins of tmRNA-mediated trans-translation in Bacillus subtilis [Fujihara et al. (2002) Detection of tmRNA-mediated trans-translation products in Bacillus subtilis. Genes Cells, 7, 343–350]. The TreP synthesis is subject to CcpA-dependent carbon catabolite repression (CCR), and the treP gene contains catabolite-responsive element (cre) sequence, a binding site of repressor protein CcpA, in the coding region. Here, we demonstrated that the tmRNA-tagging of TreP occurs depending on the gene for CcpA. In the presence of CcpA, the transcription of treP mRNA terminates at 8–9 nucleotides upstream of the 5'-edge of the internal cre sequence, and translational switch to the tag-sequence occurs at the 101st amino-acid (asparagine) position from N-terminus of TreP. The results show that trans-translation reaction is involved in the tagging and degradation of the N-terminal TreP fragment produced by truncated mRNA, which is a product of transcriptional roadblock by CcpA binding to the cre sequence in the internal coding region.

Key Words: Bacillus subtilis, CcpA, tmRNA, trans-translation, TreP

Abbreviations: CCR, carbon catabolite repression; cDNA, complementary DNA; cre, catabolite-responsive element; RT–PCR, reverse transcription & polymerase chain reaction; RACE, rapid amplification of cDNA ends; tmRNA, transfer-messenger RNA; TreP, trehalose-permease; 2D gel, two-dimensional gel electrophoresis


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. Licht and S. Brantl
The Transcriptional Repressor CcpN from Bacillus subtilis Uses Different Repression Mechanisms at Different Promoters
J. Biol. Chem., October 30, 2009; 284(44): 30032 - 30038.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.