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Journal of Biochemistry Advance Access originally published online on November 23, 2008
Journal of Biochemistry 2009 145(2):193-198; doi:10.1093/jb/mvn156
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© The Authors 2008. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

The 1.9 Å X-Ray Structure of Egg-white Lysozyme from Taiwanese Soft-Shelled Turtle (Trionyx Sinensis Wiegmann) Exhibits Structural Differences from the Standard Chicken-Type Lysozyme

Jaruwan Siritapetawee1, Sompong Thammasirirak2, Robert C. Robinson3 and Jirundon Yuvaniyama4,*

1College of Medicine and Public Health, Ubon Rajathanee University, Ubon Ratchathani 34190; 2Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand; 3Institute of Molecular and Cell Biology, Proteos, Singapore 138673; and 4Department of Biochemistry and Center for Excellence in Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

*To whom correspondence should be addressed. Tel: +66-2-201-5603, Fax: +66-2-354-7174, E-mail: scjyv{at}mahidol.ac.th

Received September 12, 2008; Accepted November 10, 2008


  Abstract

Lysozyme from Taiwanese soft-shelled turtle (SSTLB) has been purified from turtle egg white and crystallized. The crystals diffract X-rays beyond 2 Å resolution and belong to the orthorhombic P212121 space group containing one molecule per asymmetric unit. The structure was elucidated using pheasant egg-white lysozyme as the molecular replacement search template. The overall structure of SSTLB is very similar to that of hen egg-white lysozyme (HEWL). Nevertheless, Pro104 in the substrate subsite A and other amino acids in the substrate subsites E and F differ from those of HEWL. These substitutions result in local conformational differences in the substrate binding sites of the two enzymes, effecting substrate binding and transglycosylation efficiency, translating into differing ranges of products.

Key Words: crystal structure, lysozyme, soft-shelled turtle, transglycosylation, X-ray crystallography

Abbreviations: HEWL, hen egg-white lysozyme; JSSTL, Japanese soft-shelled turtle lysozyme; NAG, N-acetylglucosamine; NAM, N-acetylmuramic acid; PDB, Protein Data Bank; PEWL, pheasant egg-white lysozyme; RMSD, root-mean-square deviation; SDS–PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; SSTLA and SSTLB, Taiwanese soft-shelled turtle lysozymes A and B


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