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Journal of Biochemistry Advance Access originally published online on January 2, 2009
Journal of Biochemistry 2009 145(3):403-409; doi:10.1093/jb/mvn178
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© The Authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

TTP at Ser245 Phosphorylation by AKT is Required for Binding to 14-3-3

Shigeki Chiba, Mie Tokuhara, Eugene Hayato Morita and Shunnosuke Abe*

Laboratory of Molecular Cell Physiology, Faculty of Agriculture, Ehime University, 3-Tarumi, Matsuyama, Ehime 7908566, Japan

*To whom correspondence should be addressed. Tel: +81-89-946-9853, Fax: +81-89-946-9853, E-mail: mcblab{at}mcb.agr.ehime-u.ac.jp

Received September 17, 2008; Accepted December 22, 2008


  Abstract

Transferrin receptor trafficking protein (TTP) is a key molecule for selective internalization of the transferrin receptor (Tf-R) through endocytic protein complexes. To identify the proteins that directly regulate TTP, we performed a yeast two-hybrid analysis and identified 14-3-3, which can modulate the activation state of target proteins. Subsequent analyses demonstrated that TTP directly binds to multiple 14-3-3 isotypes via its Ser245 residue (Ser246 in human) and that these proteins are associated at the plasma membrane. Ser245 was also found to be a substrate for AKT and the resulting Ser245 phosphorylation induced the TTP–14-3-3 interaction. Exposure to hydrogen peroxide rapidly enhanced this association in an ovarian cell line. These results suggest that TTP Ser245 is the principal target for the modulation of this protein via the AKT signalling cascade.

Key Words: 14-3-3, AKT, phosphorylation, interaction

Abbreviations: Eps15, epidermal growth factor receptor pathway substrate 15; NPF, Asn-Pro-Phe; Tf-R, transferrin receptor; Ap2, clathrin adaptor protein 2; SH3, Src homology 3; EGFR, Epidermal Growth Factor Receptor; LDLR, low density lipoprotein receptor; AKT1, v-akt murine thymoma viral oncogene homolog 1; GST, glutathione S-transferase; PKC, Protein kinase C; PKA, Protein kinase A; CLK2, CDC-like kinase 2; CAMK2G, Calcium/calmodulin-dependent protein kinase II gamma; BAD, Bcl-2-associated death promoter


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