Skip Navigation


Journal of Biochemistry Advance Access originally published online on March 6, 2009
Journal of Biochemistry 2009 145(6):811-818; doi:10.1093/jb/mvp038
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow Supplementary Data
Right arrow All Versions of this Article:
145/6/811    most recent
mvp038v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Nakajima, Y.
Right arrow Articles by Nakanishi, S.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakajima, Y.
Right arrow Articles by Nakanishi, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

Isolation of ON Bipolar Cell Genes via hrGFP-coupled Cell Enrichment Using the mGluR6 Promoter

Yoshiaki Nakajima1,*, Masaki Moriyama2, Masakazu Hattori2,3, Nagahiro Minato2 and Shigetada Nakanishi1,4

1Department of Biological Sciences, Faculty of Medicine; 2Department of Immunology and Cell Biology, Graduate School of Biostudies, Kyoto University, Kyoto 606-8501; 3Department of Biosciences, School of Science, Kitasato University, Sagamihara, Kanagawa 228-8555; and 4Department of Systems Biology, Osaka Bioscience Institute, Suita, Osaka 565-0874, Japan

*To whom correspondence should be addressed. Tel: +81-75-753-4407, Fax: +81-75-753-4404, E-mail: yoshiaki{at}phy.med.kyoto-u.ac.jp

Received January 8, 2009; Accepted February 26, 2009


  Abstract

mGluR6 expression is a characteristic property of retinal ON bipolar cells. mGluR6 is also the causal gene for a form of congenital night blindness. To elucidate physiological and pathological functions of ON bipolar cells and mGluR6, we thought it important to identify genes specifically expressed in them. We thus made transgenic mouse lines expressing humanized Renilla reniformis green fluorescent protein (hrGFP), under the control of the mGluR6 promoter. From their retina, we isolated hrGFP-positive cells by cell sorting, and analysed the gene-expression profile of these cells by using DNA microarray. Further analysis revealed that about half of the initially selected ON bipolar cell genes were expressed in the expected retinal layer. We confirmed previously ambiguous retinal localization of regulator of G-protein signalling 11 (RGS11) and transient receptor potential cation channel, subfamily M, member 1 (TRPM1). In addition, we showed the expression of calcium channel, voltage-dependent, alpha2/delta subunit 3 (Cacna2d3) in ON bipolar cells for the first time. Although we could not completely exclude the possibility that a small population of hrGFP-positive cells might not be ON bipolar cells, these mice as well as our strategy would be highly valuable for the further analysis of ON bipolar cells.

Key Words: bipolar cells, Cacna2d3, mGluR6, RGS11, TRPM1

Abbreviations: Cacna2d3, calcium channel, voltage-dependent, alpha2/delta subunit 3; GAP, GTPase-activating protein; Gβ5, the fifth member of the heterotrimeric G-protein β-subunit family; hrGFP, humanized Renilla reniformis green fluorescent protein; INL, inner nuclear layer; IPL, inner plexiform layer; mGluR6, metabotropic glutamate receptor subtype 6; PI, propidium iodide; RGS11, regulator of G-protein signalling 11; TRPM1, transient receptor potential cation channel, subfamily M, member 1


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
C. W. Morgans, J. Zhang, B. G. Jeffrey, S. M. Nelson, N. S. Burke, R. M. Duvoisin, and R. L. Brown
TRPM1 is required for the depolarizing light response in retinal ON-bipolar cells
PNAS, November 10, 2009; 106(45): 19174 - 19178.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
D. Beqollari, M. J. Betzenhauser, and P. J. Kammermeier
Altered G-Protein Coupling in an mGluR6 Point Mutant Associated with Congenital Stationary Night Blindness
Mol. Pharmacol., November 1, 2009; 76(5): 992 - 997.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.