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J. Biochem, 1968, Vol. 63, No. 2 170-175
© 1968 Japanese Biochemical Society

research-article

Isolation and Properties of Ferritin from Tuna Fish (Thunnus obesus) Spleen*

TAKESHI KATO, SETSUKO SHINJO and TOSHIO SHIMADA

From the Department of Nutrition, Nippon Medical School Bunkyo-ku, Tokyo

A procedure for the isolation and crystallization of ferritin from tuna fish spleen is described and some of the physicochemical properties of this ferritin are reported. The method used for this purification were the combination of trypsin [EC 3.4.4.4 [EC] ] digestion, acetone treatment and DEAE-cellulose column-chromatography. This ferritin contained less iron and phosphorus than horse ferritin's. Isoelectric point was pH 4.3. The contents of proline, methionine and isoleucine were different from those of horse ferritin.

* Dedicated to Dr. Koozoo Kaziro, Professor Emeritus of the Nippon Medical School, on the occasion of his 70th birthday.


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