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J. Biochem, 1968, Vol. 64, No. 2 161-165
© 1968 Japanese Biochemical Society

research-article

Interaction between Bovine Pancreatic Ribonuclease and 4-Thiouridylic Acid

FUMIO SAWADA and FUMIE ISHII*

From the Chemistry Division, National Institute of Radiological Sciences Chiba

1. The interaction between bovine pancreatic ribonuclease [EC 2.7.7.16 [EC] ] (RNase) and 4-thiouridine 2'(3')-phosphate was compared with that between RNase and uridine 2'(3')-phosphate.

2. The difference spectrum of the RNase-4-thiouridylic acid complex was similar in shape to that of the RNase-uridylic acid complex, but was at about 70 µ longer wavelengths.

3. The pH dependence of the spectral change (hypochromism) of the two complexes were similar, the maxima being at between pH 5 and 6.

4. The dissociation constants of the both complexes were estimated by spectrophotometric titration to be 1.1×10–5m at pH5.6, 0.05 ionic strength and 25°G.

5. Gel filtration showed that both nucleotides bind to the enzyme in a one to one ratio.

6. These results indicate that the modes of interaction of the two nucleotides with RNase are similar.

*On leave from Kitasato University, Tokyo


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